Timo Appelhans, Felix R.M. Beinlich, Christian P. Richter, Rainer Kurre, Karin B. Busch
Here, we present a protocol for multi-color localization of single membrane proteins in organelles of live cells. To attach fluorophores, self-labeling proteins are used. Proteins, located in different membranes compartments of the same organelle, can be localized with a precision of ~18 nm.